Girish C Shukla (Girish), Ph.D.
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 Title: Associate Professor
Graduate Program Director
 Dept: Biology, Geology, Environmental Science
 Office: SR 256
 Phone: 216-687-2395,  216-523-7422
 Email: G.SHUKLA@csuohio.edu
 Address: 2121 Euclid Ave. SR 256, Cleveland, OH 44115

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Research Keywords:
Androgen Receptor, Nuclear Pre-mRNA splicing, RNA splicing, Spliceosome, U12-dependent Spliceosome
 
Education:
B.S., Biology, Delhi
Ph.D., Molecular Biology and Biochemistry, Brunel University, London
 
Brief Bio:
I teach cell biology labs and lecture.
 
Honors and Awards:
2013 Merit Award, CSU
2012 Merit Award, CSU
1996 RNA Society, University of Wisconsin
1996 Travel Award-Cooperative programs and training, International Livestock Research Institute, Nairobi, Kenya
1993-1997 Pre-doctoral fellowship, Consultative group for international agricultural research (CGIAR), World Bank, Washington, D.C.
1989-1991 Career Advancement Fellowship, International Centre for Genetic Engineering and Biotechnology, (ICGEB), and United Nation International Development Organization (UNIDO), New Delhi, India
1976-80 Delhi Administration Scholarship, Delhi Government, India
 
Research Interests:
My research goal is to better understand molecular mechanisms that control eukaryotic gene expression at the RNA level. Within this framework, I am exploring three issues: (1) noncoding RNAs and their roles in gene expression, (2) nuclear pre-mRNA splicing, and finally, (3) specific RNA:RNA and RNA:Protein interactions. The current emphasis of the lab is on miRNA-mediated regulation of human androgen receptor expression. Androgen receptor is a key modulator that has been implicated in hormonal dependent and independent stages of prostate cancer. Based on computational predictions and phylogenetic analysis I have proposed a miRNA-mediated model of Androgen Receptor regulation. One particularly interesting idea that I would like to investigate further is the critical level of Androgen receptor influences the transition from the hormone-dependent to hormone refractory stage of prostate cancer. Using computational biology tools I have identified a number of miRNAs that have the potential to interact with the 3' untranslated region of the receptor mRNA. This observation gives rise to the hypothesis that androgen receptor expression is tightly regulated by combinatorial control of miRNAs and deregulation of these miRNAs may contribute in the progression of prostate cancer. Using lentiviral expression systems we are overexpressing a number of miRNAs to understand how androgen receptor expression is controlled by these miRNAs in a variety of prostatic carcinoma cells.

The research in my laboratory is also aimed at understanding the nuclear pre-mRNA splicing by using in vitro and in vivo methods. We are currently studying the structure-function of several snRNAs of the minor spliceosome including U11, U12, U4atac and U6atac. Recently we have identified a small RNA element in the U6atac snRNA that functions in the 'guiding' of the specific spliceosomal complexes to the minor class splice sites. We are now pursuing the identification of proteins that may interact with these U6atac snRNA sub-structures to further define their role in the splicing of minor class introns. We are also studying specific RNA-RNA base-pairing interaction involved in the splicing of the minor class intron using biochemical and in vivo genetic approaches. These studies will help us to understand how multiple sequentially interacting snRNAs facilitate the removal of introns from the precursor mRNA of genes.
 
Teaching Areas:
Cell Biology, Cancer Biology, Molecular Biology with specialty in RNA Molecular Biology
 
Professional Affiliations:
The RNA Society
American Association for Cancer Research
American Association for the Advancement of Science
American Society for Microbiology
Member, GU malignancies program, Case Comprehensive Cancer Center
 
University Service:
2007-2009 Faculty Senate
2007-2011 Undergraduate Curriculum and Academic Standards  Committee
2007-2009 Member Student Life Committee
2007-2010 Member Honors Council
2007-2010 Member Recreation Center Committee
2008-2009 Member COS Curriculum Committee
2010-2012 COSHP Budget Committee
2010 Invited by the RNA Society to Mentor students and postdoctoral fellows at the RNA Meeting, Seattle, Washington.
2012 Invited by the RNA Society to Mentor students and postdoctoral fellows at the RNA Meeting, Ann Arbor, Michigan.
2010- COSHP Ad-hoc Strategic Planning Committee
2011- Graduate Curriculum and Academic Standards  Committee
2011-2013 Graduate Council and Graduate Faculty Status Sub-committee
2014 Reviewer summer engaged learning grant CSU
2013 Graduate program director
 
Professional Service:
GRANTS STUDY SECTION ASSIGNMENTS
Ad Hoc Grants Reviewer
Congressionally directed medical research program, Department of Defense
2006: Breast Cancer Research Program, Pathobiology (11 grants)
2006: Prostate cancer Research Program, Cell and Molecular Biology
2007: Prostate cancer Research Program ┬┐Training grants
2008: Breast Cancer Research Program
Cell Biology Study section
Molecular Biology and Genetics Study Section
Pathobiology Study section
2008: American Heart Association
2009: American Heart Association
2009: Breast Cancer Research Program
2010: Prostate Cancer Research Program Cell Biology Study Section
2010: Prostate Cancer Research Program Cell/Molecular Biology Section (Six Grants)
2011: Prostate Cancer Research Program Biomarker discovery (12 Grants)
2012: Prostate Cancer Research Program Cellular and Molecular Biology panels (15 Grants)
2013: Declined the invitation to serve the panel
2013: Prostate Cancer Research Foundation of the United Kingdom

2010-2012:Panel Member in Molecular and Cellular Biology, Genes and Genome Cluster, National Science Foundation


Scientific Journals Ad Hoc Reviewer
International Journal of Cancer, PNAS, Oncogene, Nucleic Acid Research, RNA, Frontiers of Biosciences, World Journal of Oncology, Molecular  Biotechnology, Carcinogenesis, Molecular Biosystems,  Colloids and Surfaces, Journal of Carcinogenesis, PLoS one, PLoS Genetics, Molecular Carcinogenesis, Molecular Cancer

National Meeting Chair and Co-chair
1. 2008 Co-chair Rust Belt RNA Meeting, Deer Creek, OH
2. 2009 Chair Rust Belt RNA Meeting, Deer Creek, OH
 
Community Service:
COMMUNITY SERVICE
 2005 Intel Science fair grand award judge
 2007 Concept School Science Fair Judge
 2008 Special Award Judge NEOSEF
 2008 Concept School Science Fair Judge
 2009 Special Award Judge NEOSEF
 2009 Concept School Science Fair Judge
 2010 North East Ohio Science and Engineering Fair Special Award Judge
 2013 Volunteer for Urban Squash Program
 
Research Grants:
2014 Department of Defense (Prostate Cancer Research Program)
Novel Roles of Androgen receptor by controlling noncoding RNA maturation to promote castration resistant prostate cancer.
2014 Department of Defense (Prostate Cancer Research Program)Precursor miRNA binding oncogenic proteins in prostate carcinogenesis
PENDING
Title of the project: Assembly of RNA nanoparticles for target delivery to prostate cancer cells Application ID: NIH (Institute: NIBIB) 2R01EB003730-10; NIH Appl. ID: 8761691
PI: Guo, Peixuan (pi); Shukla, Girish C (Co-PI); St. Clair, William H (Co-PI)

IRG-91-022-12 2005-2006 American Cancer Society and Case Comprehensive Cancer Center
Roles of alternatively spliced variants of a tumor suppressor WW domains containing oxidoreductase (WWOX) in prostate cancer

2006-2009 Department of Defense (Prostate Cancer Research Program)
Targeting androgen receptor function by microRNA in prostate cancer $135,000

National Science Foundation (PI: Shukla) 10/2009-10/2010
This grant was awarded to partially support regional RNA meeting known as Rust Belt RNA Meeting. I was the coorganizer for 2008 meeting. I will be the organizer of 2009 meeting. Details can be found at www.rustbeltRNA.org.  [Awarded at CSU] Total Direct Cost - $7,205.00

2009 ┬┐ 2011 Department of Defense (Breast Cancer Research Program)
Splicing Interference (SPLICEi) by Small Nuclear RNAs in Breast Cancer $106,500

2010-11 Ohio Board of Reagents/Scholars Cardiac Myosin Heavy Chain Alpha and and hsa-miR 208a Processing $60,000

2011-13 Department of Defense (Prostate Cancer Research Program)
MicroRNA Targets of Human Androgen Receptor $106,500

2009-13 National Science Foundation
U12-dependent spliceosomal snRNA $368,363
2012: COMPETITIVE INVITATION declined)Department of Defense (Prostate Cancer Research Program)
Inactivation of Astrotactin I (ASTNI) a Potential tumor Suppressor Gene in Prostate Cancer