Jacqueline Vitali, Ph.D.
 Title: Assoc Prof Physics and BGES
 Dept: Physics
 Office: SI 122
 Phone: 216-687-2431
 Email: J.VITALI@csuohio.edu
 Address: 2121 Euclid Ave. SI 122, Cleveland, OH 44115

Courses Taught

Publications


Faculty Only:
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Research Keywords:
Crystallography, x-ray Diffraction, Proteins, Drug discovery, Extremophiles, Archaeal Proteins, Proteins of the Pyrimidine Pathway, Ornithine Transcarbamoylase, Aspartate Transcarbamoylase, Regulatory Enzymes
 
Education:
Ph.D., Biophysics, State University of New York at Buffalo
B.S., Physics, University of Athens
 
Brief Bio:
will come soon
 
Creative and Activities:
Very recent only --

Vitali, J., Singh, A.K., Soares, A.S. and Colaneri, M. (2012) Structure of the catalytic chain of Methanococcus jannaschii aspartate transcarbamoylase in a hexagonal crystal form. Insights into the path of carbamoyl phosphate to the active site of the enzyme. Amer. Cryst. Assoc. Annual meeting at Boston, MA. Abstract M-24, poster presentation.

Vitali, J. (6/1/2012) Structure of the catalytic subunit of M. jannaschii aspartate transcarbamoylase. 42nd Mid-Atlantic Macromolecular Crystallography Meeting, Charloteesville, VA. Oral presentation.
 
Research Interests:
      My research involves the structural analysis of macromolecules by X-ray diffraction.  Such studies provide direct and unambiguous information regarding all the details of the three-dimentional atomic arrangement of these molecules.  This information is necessary in order to understand their properties and function.
      My current efforts focus in acquiring a better understanding of de novo pyrimidine biosynthesis at the molecular level.  In this connection, I have determined the structure of the catalytic trimer of the enzyme Aspartate transcarbamoylase from the hyperthermophilic and barophilic archaeon M.jannaschii in two crystal forms.  This research gave insight into the strategies for thermostabilization of this important enzyme.  The most intriguing feature of these structures is the association of crystallographically independent subunits in pairs into a novel staggered complex with a short inter-trimer distance of 33.8 Å. This novel staggered arrangement may be present in the holoenzyme in vivo in the presence of the regulatory subunits.  In order to gain more insight into the mechanism of catalysis we co-crystallize the enzyme with substrate analogs.
      Past research includes complexes of bovine thrombin with inhibitors including the leech protein hirudin, one Fab fragment, S100b, the EF-Tu-Ts complex, pectin lyase B, histidine decarboxylase, the Gly121Tyr mutant of ornithine decarboxylase from Lactobacillus 30a, the UP1 fragment of hnRNP A1, myoglobin, and insulin.
      
Postdoctoral fellow:
Aditya Singh, Ph.D., 10/2007-3/2009
 
Teaching Areas:
Algebra and Calculus based Physics
Biophysics
Crystallography

Undergraduate Research Students
Oana Sipos, 1/2004 - 5/2007
Nermina Covic, 7/2005 - 11/2006
Khadidja Benmerzouga, 4/2007 - 9/2009
Frank Marealle, 3/2008 - 5/2008
Jonathan Allen, 7/2008 - 9/2008
Nura Orra, 1/2009 - 9/2009
Charlie Dorflinger, 6/2010 - 9/2010
        
Graduate Research Students
Dan Meleason, Fall 2008

Graduate Student Committees
Baixi Su-Alexander (Electrical Engineering, D.E. 5/2008):
Ozge Can (Chemical and Biomedical Engineering, D.E. 12/2008):
Boo Seok Yun (Clinical Chemistry, Ph.D. program)
 
Professional Affiliations:
American Crystallographic Association
 
University Service:
Environmental Health and Safety Advisory Board, 9/12-present
Physics Peer Review Committee, 1/12 - present
Equal Opportunity Hearing Panel, 10/11 - present
Curriculum Committee in Medical Physics, 9/11 - present
Graduate Grade Dispute Committee, 8/09 - 10/11                  
Graduate Faculty Review Committee, 8/07 - 8/09
Graduate Admissions and Standards Committee, 8/05 - 8/07
Steering Committee in Medical Physics, 1/03 - present
Physics Faculty Search Committee, 2003 - 2004
Physics Faculty Search Committee, 2005 - 2006
Physics Faculty Search Committee, 2008 - 2009
Physics Visiting Faculty Search Committee, 4/12 - 5/12
 
Professional Service:
      Reviewer for Acta Crystallographica, Biochemistry, Bioorganic and Medicinal Chemistry, Accounts of Chemical Research, Archives of Biochemistry and Biophysics.
      Chair of poster session on Biological Macromolecules in the 1994 Amer. Cryst. Assoc. Annual Meeting at Atlanta, Georgia
      Chair of the Theory Session of Contributed Papers in the "2005 World Year of Physics Einstein Centennial: From Brownian Motion to the Physics of Complexity" meeting of the Ohio Section of the American Physical Society at Cleveland State University, Cleveland, Ohio (10/15/2005).
 
Research Grants:
      Structural Studies of an Aspartate Transcarbamoylase.  Faculty Research Development Award; 7/09-12/10 (CSU). Amount: $15K.
      Structural Studies of the Pyrimidine Pathway.  NIH R15 grant; 9/04-9/09 (CSU). Amount: $210K.
      Engaged Learning Undergraduate Program; summer 2010, summer 2009, summer 2008; ~ $4K per summer, Office of the Provost, CSU.
      Startup Package Fund (CSU)
      National Research Service Award from institutional training grant in experimental hematology; 9/92-7/94.  (WSU)
      Determination of the Crystal Structure of the S100b Protein.  Cystic Fibrosis Foundation Research Fellowship; 5/87-5/89. (UVa)