David J Anderson (Dave), Ph.D.
 Title: Professor
Director of Clinical Chemistry
 Dept: Chemistry
 Office: SR 366
 Phone: 216-687-2453
 Fax: 216-687-9298
 Email: D.ANDERSON@csuohio.edu
 Address: 2121 Euclid Ave. SR 366, Cleveland, OH 44115

Courses Taught


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Research Keywords:
High performance liquid chromatography, mass spectrometry, novel HPLC techniques, gradient chromatofocusing, applications of HPLC-MS of biological compounds, proteins, gangliosides, peptides, dynorphins, drugs, chromatography theory and mechanisms, disease markers and etiology studies in glaucoma and hearing loss
Post-Doctorate, Clinical Chemistry, Mayo Clinic Foundation, 1988
Ph.D., Analytical Chemistry, Iowa State University, 1986
B.S., Chemistry, Bucknell University, 1977
Brief Bio:
Dr. David Anderson is Professor of Chemistry at Cleveland State University and Director of Clinical Chemistry at CSU.

The doctoral clinical chemistry program is a nationally and internationally recognized program accredited by the Commission on Accreditation in Clinical Chemistry, the only accredited doctoral program in the US. This field prepares leaders in the clinical laboratories, both within the medical institutions as directors of clinical laboratories and as clinical chemists in the industries of diagnostic medicine, as well as other related industries, such as the pharmaceutical sector. This program has awarded over 100 doctorates. Graduates from our doctoral program are directors of clinical laboratories at major medical facilities including the Mayo Clinic, Yale, Duke, Vanderbilt, Tulane, University of Cincinnati and others. Ph.D. graduates from this program are also directors of Cleveland clinical laboratories at Louis Stokes Cleveland VA Medical Center, MetroHealth Medical Center and Cleveland HeartLab. Graduates have attained high level positions, including vice president and CEO positions.

Dr. Anderson's research involves developing sophisticated HPLC and mass spectrometry techniques for the measurement and characterization of proteins, lipids, and drug compounds. His group also studies fundamental aspects of chromatographic separations.
Honors and Awards:
Certified by American Board of Clinical Chemistry, Diplomat in Clinical Chemistry, 1992-Present

2020 Merit Award, Faculty, Cleveland State University, 2020

2019 Distinguished Faculty Teaching Award, Cleveland State University, 2019

2017 Jearl D. Walker Outstanding Teaching Award, College of Sciences and Health Professions, Cleveland State University,  2018

Merit Award, Faculty, Cleveland State University, 2017

Apple Polishing Award, Dedicated Service to Students, Order of Omega Honor Society,
Cleveland State University, Academic Year 2016-2017

Golden Apple Award, Young Alumni Council, Cleveland State University Alumni Association,

Merit Award, Faculty, Cleveland State University, 2014

Merit Award, Faculty, Cleveland State University, 2013

Merit Award for Administrative Faculty, Cleveland State University, 2008

Travel Award, 11th International Bioanalytical Forum, Guildford, United Kingdom,
August 29 - Sept 1 1995.

Van Slyke Clinical Chemistry Award, American Association for Clinical Chemistry, 1993

Travel Grant, National Science Foundation, Eighth International Symposium on Affinity Chromatography and Biological Recognition, Jerusalem, Israel 1989.
Research Interests:
LC-MS/MS Profiling of Gangliosides
Gangliosides are implicated in various neurological diseases including: Alzheimer¿s, Parkinson¿s and Huntington¿s diseases. A novel LC-MS/MS technique (2021 publication) separated bovine and mouse brain gangliosides GM1, GD1a, GT1b and GQ1b using a phenyl-hexyl HPLC column. The gangliosides are separated according to sialic acid class, and within the particular class, gangliosides having different ceramide carbon chain lengths are also separated. This technique is advantageous over the currently used reversed-phase or HILIC techniques, which can only separate according to ceramide or sialic acid content, respectively.  In addition, a LC-MS/MS was developed (2015 publication) to quantify individual molecular components of gangliosides, as opposed to current techniques which are limited to determining ganglioside subtypes (multiple structures with the same MW). I am the corresponding author of the publications below. I have been invited, and have accepted, to write a review for the 60th volume of Advances in Chromatography on the topic ¿New advances for separation of gangliosides¿.

Dynorphin-mediated inner ear immune/inflammatory response and glutamate-induced neural excitotoxicity
Application of liquid chromatography-mass spectrometry (LC-MS/MS) in the determination of dynorphin peptides was done. A technique has been developed that determines dynorphins at physiologic levels which has not been previously accomplished. This will be applied to investigate the role of dynorphins in immune inflammatory response in inner ear inflammatory/immune disorders.

Gradient Chromatofocusing
Our group has pioneered a new charged-based HPLC technique called gradient chromatofocusing that separates proteins according to their pI using linear pH gradients. Conventional chromatofocusing utilizes polymeric ampholyte buffers employing a step change gradient to generate the linear pH gradient. Many disadvantages of the conventional technique are addressed by our gradient chromatofocusing techniqu. Gradient chromatofocusing results showed significantly better resolution of proteins compared to conventional chromatofocusing and salt gradient ion-exchange HPLC. With this technique proteins are identified both by elution pH related to their pI and by the MW determined by mass spectrometry.

LC-MS/MS Method Development and Validation Studies of Newly-Developed Drug Agents in Biological Samples
LC-MS/MS MRM methods were developed and evaluated according to FDA guidelines for novel neurodegenerative drug candidates determined in mouse serum and brain samples. Methods were evaluated for precision, recovery, linearity, matrix effect, stability and carryover and were found to meet FDA guidelines.

Fundamental aspects of chromatographic separations
Application of polarity models to characterizing the reversed-phase liquid chromatography separation of chiral steroid isomer pairs employing various ternary mobile phases.
Teaching Areas:
Graduate courses in clinical chemistry, graduate course in HPLC, undergraduate courses in college chemistry for pre-nursing and health science majors
Professional Affiliations:
Member, American Association for Clinical Chemistry
Certified by American Board of Clinical Chemistry, Diplomat in Clinical Chemistry, 1992-Present
Professional Experience:
1988-1993 CSU, Dept. of Chemistry- Assistant Professor, Chemistry
1992-Present, American Board of Clinical Chemistry, Diplomat in Clinical Chemistry
1992-Present CSU, Dept. of Chemistry- Director/Co-Director, Clinical Chemistry
1994-2003 CSU, Dept. of Chemistry- Associate Professor, Chemistry
1998-Present Cleveland Clinic - Adjunct Staff, Lerner Research Institute

1997-1999 CSU, Dept. of Chemistry- Head, Mass Spectrometry Facility
1999- 2005 CSU, Dept. of Chemistry- Director, Mass Spectrometry Facility
2003-Present CSU, Dept. of Chemistry- Professor, Chemistry
2005 CSU, College of Science- Interim Associate Dean
2006-2010 CSU, College of Science/Sciences and Health Professions- Associate Dean
2010-2012 CSU, Dept. of Chemistry- Chair
2013- present Northeast Ohio Medical University-Dept. of Pharmaceutical Sciences A
Adjunct Professor
University Service:
Current, Department of Chemistry

Director/Co-Director, Clinical Chemistry (1991- Present) (Director 1991-2006, 2013-present)
Chair, The Fred E. Sheibley Charitable Trust Committee, 2016-present;Member (2013 ¿ 2015)
Chair, Steering Committee (2013-present)
Member, Graduate Committee (1991-2005, 2010, 2021-present)
Mentor, New Faculty, Yana Sandlers (2015-present)

Recent Past, Department of Chemistry

Principal Coordinator and Writer, Chemistry Program Review Self-Study (2021)
Member, Organic Chemistry Faculty Search Committee (2021-2022)
Member, Research Scientist Search Committee (2021)
Head, Chemistry Chair Search (2017)
Contributor, Self Study of Chemistry Department (1991-92, 2014-15)
Chair, Faculty Search Committee, Assistant Professor of Clinical Chemistry (2014)
Chair, Faculty Search Committee, Assistant Professor of Clinical Chemistry (2013)
Faculty Search Committees (Chair of 4, Member of 4, 1989-2000), several hires

Past, College of Sciences and Health Professions(College of Science)

Chair, College Peer Review Committee (2019-2022)
Member, Peer Review Committee for Promotion to Professor, Physics, (2015-2018)
Member, College Curriculum Committee (2013-2015)
Member, College Reorganization Committee (2002) (College of Arts and Science)
Member, College Peer Review Committee (2004-2005; 2018-2019)
Chair, Search Committee, Manager of Student Affairs (2005), hired Kristy Tokarczyk
Chair, Search Committee, College of Science Budget Manager (2007), hired Luanne Merner

Past, University

Coordinator, Biomedical Research Cleveland (1997-2001)
Represented Provost at high level meetings with Cleveland Clinic, Case Western Reserve University, University Hospitals, and MetroHealth Medical Center
Member, Advisory Committee, Center for Health Sciences and Human Services (1994 - 1999)
Member, CSU/CCF Inter-institutional Committee (1996 -2000)
Member, Retention Steering Committee (2006)
Member, Student Value and University Enrollment Initiative (2006)
Member, President¿s Retention Committee (2006-2008)
Member, University Strategic Planning Committee (2006 ¿ 2008)
Member, Assessment Council (2005-2010)
Member, NCA Self Study, Criterion 5 Sub-committee (2008-present)
NCA Self Study, Rewrote Criterion 4 (2010)
Professional Service:
American Association for Clinical Chemistry, Northeast Ohio
Chair  (1999 - 2002) , Membership Chair (1996 - 2005), Treasurer (2006-present)

Clinica Chimica Acta, Member, Editorial Board 1999 - 2005
Research Grants:
Selective Funded Grants

Grant-in-Aid, American Heart Association, Northeast Ohio Affiliate, Determination of
Fibrin I and Fibrin II Monomers in Plasma (PI D. Anderson), 1991-1992, $20,111.

Grant-in-Aid, Continuation, American Heart Association, Northeast Ohio Affiliate,
Determination of Fibrin I and Fibrin II Monomers in Plasma (PI D. Anderson), 1992-
1993, $8,815.

Van Slyke Society Research Grant, American Association for Clinical Chemistry (with
M. Ip, PI D. Anderson), Determination of Glycosylated Variants of AFP Using High-
Performance Lectin Affinity Chromatography and On-Line Immunodetection in the
Diagnosis of Hepatocellular Carcinoma, 1993-1994, $5,000.

State of Ohio Capital Appropriations to Biomedical Research Cleveland and The Cleveland Clinic Foundation, Cleveland Mass Spectrometry Facility, (PI David Anderson), 1997,  $260,000.
Promoting Research Initiatives with Major External Sponsors (PRIMES), Cleveland State University, Analytical Research Center for Biomolecular Structure, (Co-PI D. Anderson) 1997-1999, $150,000
Gradient Chromatofocusing-Mass Spectrometry (GCF-MS) in Proteomics, Faculty Research Enhancement Award,  College of Arts and Sciences, Cleveland State University, PI David Anderson, June 1, 2001 ¿ May 15, 2002, $3000.
Mass Spectrometry Consortium for Material and Medical Research, Hayes Investment Program, Ohio Board of Regents, PI Chrys Wesdemiotis (Akron), D. Anderson Co-PI (CSU PI), $1,870,000 (CSU $180,000), June 2002 ¿ May 2005.
Mechanisms of Tubular Atrophy in Renal Disease, R01, National Institutes of Health, PI Jeffrey Schelling (MetroHealth), D. Anderson subcontract PI, August 2006 ¿ July 2008, $92,566 (subcontract).
GAANN Chemistry Scholars, Graduate Assistance in Areas of National Need, U.S. Department of Education, PI Yan Xu, Co-PI David Anderson, 2007-2010, $383,643.
Major Research Instrumentation, MRI: Acquisition of an LC-MS/MS system by Cleveland State University, National Science Foundation, Yan Xu PI, David Anderson Co-PI, 2009 ¿ 2012, $514,000
LC-MS/MS Profiling of Gangliosides in Retina: Application to Glaucoma, Faculty Research Development, Cleveland State University, David Anderson, PI, July 2013 ¿ December  2014, $24,870
Establishing Locus Coeruleus Activation as a Mechanism of Dynorphin Release in the Brain, Faculty Scholarship Initiative, Cleveland State University, David Anderson, PI, May 2020-May 2022, $5000
Mouse Neurosurgery, ELISA and Cryostat Histology for Corticosterone and Dynorphin B, Undergraduate Summer Research Award, Cleveland State University, M. Hammonds PI, T. Sahley CoPI, D. Anderson CoPI, May ¿August 2021, $4137.88
Mouse Neurosurgery, ELISA and Cerebrospinal Fluid Extraction for Cortisone and Dynorphin B Undergraduate Summer Research Award, Cleveland State University, M. Hammonds PI, T. Sahley CoPI, D. Anderson CoPI, May ¿August 2022, $4800.80