| Title: |
Assistant Professor
Consultant, Dept. of Cardiovascular and Metabolic Sciences, LRI, Cleveland Clinic-OH |
| Dept: | Biological, Geological and Environmental Sciences |
| Office: | SI 319 |
| Phone: | 216-687-2409 |
| Email: | k.gulshan@csuohio.edu |
| Address: | 2121 Euclid Ave. SI 319, Cleveland, OH 44115 |
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| Education: | ||||||||
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Ph.D., Glutathione mediated detoxification pathways, Jawahar Lal Nehru University (JNU) New Delhi
Postdoc, Oxidative stress & Multidrug resistance, University of Iowa, Iowa city, USA, 2011 Project Scientist, Cholesterol and inflammation in cardiovascular disease, Cleveland Clinic, 2020 | ||||||||
| Brief Bio: | ||
| Our research is focused on the role of inflammation in cardiovascular disease (CVD)and lung cancer. In the past several years, I described the role of free cholesterol in inducing autophagy and sphingomyelin biosynthesis in macrophages (PNAS 2015, Autophagy 2015). I also discovered a novel role for a minor phospholipid PIP2 in HDL biogenesis, determining that PIP2 is the lipid recognized by apoA1 in ABCA1-expressing cells, and identifying ABCA1 as a first known PIP2 floppase(Circulation Research 2016). This novel PIP2 floppase activity of ABCA1 was later confirmed by multiple studies from other labs. We identified novel anti-atherosclerotic activities of the anti-protozoan drug Miltefosine (Scientific Reports, June 2019). More recently, we demonstrated role of inflammatory protein Gasdermin D (GsdmD) in atherosclerosis by using LDLr Antisense Oligonucleotide (ASO) KO mouse model of hyperlipidemia. We showed for the first time that whole body KO of GsdmD protects against inflammation-induced defects in reverse cholesterol transport (RCT) and atherosclerosis. We showed for the first time the presence of cleaved N-terminal fragment of GsdmD in atherosclerotic plaques (Front.Cell.Dev.Biol, 2021).
My lab at the Cleveland State University is equipped with BD Flow cytometer, Leica Microtome for tissue sectioning, QuantStudio 3 Real-Time PCR machine (ThermoFisher), SystemIBRIGHT CL750, Imaging System (Life technologies), CountessTM 3 FL Automated Cell Counter (Life technologies), bright & fluorescent microscopes for images and videos, various centrifuges, and all other standard lab equipment. We employ cutting edge technologies such as Crispr-Cas9, RNA Seq, 10X Single cell RNAseq, STED/STORM microscopy, 16s rDNA seq, multiplex cytokine arrays and various mouse models of atherosclerosis and lung cancer to decipher the role of cholesterol and minor phospholipids in inflammasome-induced CVD and lung cancer. The lab personnel includes two Postdocs, 4 PhD candidate graduate students, and a technician. Our research is funded by NIH RO1 grant, American Heart Association Transformational Project Award to study the role of PIP2 in atherosclerosis and inflammation. Lung cancer project is funded by Case Comprehensive Cancer Center pilot grant from Case Western University to study the role of inflammation in lung cancer progression and metastasis. I also contribute to atherosclerosis and inflammation field by performing peer review for over 30 scientific journals, such as Cell death & disease, JAHA, Atherosclerosis, Autophagy, iScience, Scientific reports, Cytokine, Clinical and Experimental Allergy,Inflammatory Bowel Diseases, Cardiovascular & Hematological Disorders-Drug Targets, Drug Design, Development and Therapy. I also serve as an editor for Biomarkers of Cardiovascular Diseases; Disease Marker and associate/guest editor for several journals. In addition to research, I have experience of multiple years in teaching Microbiology with Lab, Pathophysiology, and biochemistry courses. I also serve as an Adhoc reviewer for NIH-NHLBI study sections (INMP, NMHD, 2020-current). | ||
| Honors and Awards: | ||
| Honors/awards:
-American Heart Association Transformational Project Award (2023-2026). -Cleveland State University Merit award for research, 2023. -2022 Outstanding Associate Editor, Frontiers in Cardiovascular Medicine -Case Comprehensive Cancer Center (NCI) award (2020-21) -Elsa Albretch award for best paper, 2019, Lerner Research Institute, Cleveland Clinic -Elsa Albretch award for best paper, 2017, Lerner Research Institute, Cleveland Clinic. -Best poster award, Gordon Research Conference of lipoprotein metabolism 2016. -Best poster award, Cleveland Clinic research day 2016 (1st prize). -NCAI-Cleveland Clinic-I-Corps award (2016) -Best poster award, Lerner Research Institute research day 2016 (1st prize). -American Heart Association scientist development grant award (2015-2019). -Best poster award, Lerner Research Institute research day 2015 (2nd prize). -Best poster award, Cleveland Clinic research day 2012 (1st prize). | ||
| Research Interests: | ||
| 1) Role of Sphingolipids in Cardiovascular disease.
2) Autophagy in heart disease and cancer. 3) Role of inflammation in heart disease. 4) Role of inflammation in lung cancer. | ||
| Teaching Areas: | ||
| Microbiology, Pathophysiology, Biochemistry. | ||
| Professional Affiliations: | ||
| 2016-current- Member, American Heart Association (AHA)
2021-current -Member, American Society of Biochemistry and Molecular Biology (ASBMB) | ||
| Professional Experience: | ||
| 2005-2011: Assistant Research Scientist, University of Iowa, Iowa-city, IA.
2011- 2015:Research Associate, Lerner Research Institute, Cleveland Clinic, OH 2015-2016:Project Scientist, Lerner Research Institute, Cleveland Clinic, OH 2016-2020: Adjunct Assistant Professor of Molecular Medicine, Cleveland Clinic, OH | ||
| University Service: | ||
| Member of CSU IACUC committee (2021-current).
Teaching Biochem courses (2020-current) | ||
| Professional Service: | ||
| Grant review for NIH, and manuscripts review for various peer-reviewed journals. | ||
| Community Service: | ||
| Judging scientific posters/events:
Annual Concept Science & Engineering Fair (CONSEF), Cleveland (2014-2017). NEOSEF, Cleveland State University (2016). Solon Middle School Science Fair, Solon, OH (2017) | ||
| Research Grants: | ||
| Active:
1) NIH-NHLBI-R01 HL148158 Gulshan (PI) 04/01/20-03/31/24 Phosphatidylinositol metabolism and trafficking in atherosclerosis and inflammation. 2) American Heart Association- 23TPA1063910 Gulshan (PI) 07/01/23-06/30/26 Role of cholesterol mediated modulation of PIP2 in cardiovascular disease. Completed: 1) Case Comprehensive Cancer Center: (K Gulshan, PI) (06/2020-06/2021) Title: Role of PIP2 and Gasdermin D in Lung Cancer Progression and Metastasis. 2) American Heart Association Scientist Development Grant SDG25710128 (K Gulshan, PI) (7/1/2015-6/30/2019) Title: Mechanisms of cellular lipid homeostasis and nascent HDL formation. 3) NCAI-CC-2016-I-Corps-SD (K Gulshan, PI) (6/1/2016-7/31/2016). | ||