A Glycopolymer Chaperone for Fibroblast Growth Factor-2

Ran Guan; Xue Sun; Sijian Hou; Peiyi Wu; Elliot L. Chaikof

doi:10.1021/bc034138t

A Glycopolymer Chaperone for Fibroblast Growth Factor-2

Ran Guan
, Xue Sun
, Sijian Hou
, Peiyi Wu
, Elliot L. Chaikof

Chemistry

Emory University School of Medicine
Georgia Institute of Technology
Emory University

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Mono- and disaccharide-containing glycopolymers were synthesized by cyanoxyl-mediated polymerization of acrylamide with acrylate-derivatized mono- and disaccharides. We demonstrate that a glycopolymer bearing pendant, fully sulfated lactose units effectively replaces heparin and heparan sulfate as a molecular chaperone for fibroblast growth factor-2 (FGF-2). Specifically, a compound was identified that protects FGF-2 from proteolytic, acid, and heat-induced degradation, while selectively promoting growth factor and receptor dimerization. Significantly, the capacity of this heparin-mimic to promote an FGF-2 specific proliferative cell response was confirmed and suggests potential applications for this compound and related derivatives in areas related to therapeutic angiogenesis.

Original language	English
Pages (from-to)	145-151
Number of pages	7
Journal	Bioconjugate Chemistry
Volume	15
Issue number	1
DOIs	https://doi.org/10.1021/bc034138t
State	Published - Jan 1 2004

Access to Document

10.1021/bc034138t

Cite this

@article{9f81ea0ca68f4744aa69051a43aab84b,

title = "A Glycopolymer Chaperone for Fibroblast Growth Factor-2",

abstract = "Mono- and disaccharide-containing glycopolymers were synthesized by cyanoxyl-mediated polymerization of acrylamide with acrylate-derivatized mono- and disaccharides. We demonstrate that a glycopolymer bearing pendant, fully sulfated lactose units effectively replaces heparin and heparan sulfate as a molecular chaperone for fibroblast growth factor-2 (FGF-2). Specifically, a compound was identified that protects FGF-2 from proteolytic, acid, and heat-induced degradation, while selectively promoting growth factor and receptor dimerization. Significantly, the capacity of this heparin-mimic to promote an FGF-2 specific proliferative cell response was confirmed and suggests potential applications for this compound and related derivatives in areas related to therapeutic angiogenesis.",

author = "Ran Guan and Xue Sun and Sijian Hou and Peiyi Wu and Chaikof, \{Elliot L.\}",

year = "2004",

month = jan,

day = "1",

doi = "10.1021/bc034138t",

language = "English",

volume = "15",

pages = "145--151",

journal = "Bioconjugate Chemistry",

issn = "1043-1802",

publisher = "American Chemical Society",

number = "1",

}

TY - JOUR

T1 - A Glycopolymer Chaperone for Fibroblast Growth Factor-2

AU - Guan, Ran

AU - Sun, Xue

AU - Hou, Sijian

AU - Wu, Peiyi

AU - Chaikof, Elliot L.

PY - 2004/1/1

Y1 - 2004/1/1

N2 - Mono- and disaccharide-containing glycopolymers were synthesized by cyanoxyl-mediated polymerization of acrylamide with acrylate-derivatized mono- and disaccharides. We demonstrate that a glycopolymer bearing pendant, fully sulfated lactose units effectively replaces heparin and heparan sulfate as a molecular chaperone for fibroblast growth factor-2 (FGF-2). Specifically, a compound was identified that protects FGF-2 from proteolytic, acid, and heat-induced degradation, while selectively promoting growth factor and receptor dimerization. Significantly, the capacity of this heparin-mimic to promote an FGF-2 specific proliferative cell response was confirmed and suggests potential applications for this compound and related derivatives in areas related to therapeutic angiogenesis.

AB - Mono- and disaccharide-containing glycopolymers were synthesized by cyanoxyl-mediated polymerization of acrylamide with acrylate-derivatized mono- and disaccharides. We demonstrate that a glycopolymer bearing pendant, fully sulfated lactose units effectively replaces heparin and heparan sulfate as a molecular chaperone for fibroblast growth factor-2 (FGF-2). Specifically, a compound was identified that protects FGF-2 from proteolytic, acid, and heat-induced degradation, while selectively promoting growth factor and receptor dimerization. Significantly, the capacity of this heparin-mimic to promote an FGF-2 specific proliferative cell response was confirmed and suggests potential applications for this compound and related derivatives in areas related to therapeutic angiogenesis.

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0842306886&origin=inward

UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=0842306886&origin=inward

U2 - 10.1021/bc034138t

DO - 10.1021/bc034138t

M3 - Article

C2 - 14733594

SN - 1043-1802

VL - 15

SP - 145

EP - 151

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

IS - 1

ER -

A Glycopolymer Chaperone for Fibroblast Growth Factor-2

Abstract

Access to Document

Other files and links

Cite this