A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)

Alisha House; Erica Fatica; Rohan Shah; Jared Stergar; Ryan Pearce; Yana Sandlers

doi:10.1016/j.mex.2019.05.028

A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)

Alisha House
, Erica Fatica
, Rohan Shah
, Jared Stergar
, Ryan Pearce
, Yana Sandlers

Cleveland State University

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Recent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here we report a step by step protocol for iPSCM metabolic characterization. The described assays cover analysis of small metabolites involved in a vital metabolic pathways.

Original language	English
Article number	100572
Journal	MethodsX
Volume	7
DOIs	https://doi.org/10.1016/j.mex.2019.05.028
State	Published - Jan 1 2020

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

GCMS
Induced pluripotent stem cell-derived cardiomyocytes
LC–MS/MS
Metabolic characterization
iPS-CM functional characterization

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10.1016/j.mex.2019.05.028

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abstract = "Recent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here we report a step by step protocol for iPSCM metabolic characterization. The described assays cover analysis of small metabolites involved in a vital metabolic pathways.",

keywords = "GCMS, Induced pluripotent stem cell-derived cardiomyocytes, LC–MS/MS, Metabolic characterization, iPS-CM functional characterization",

author = "Alisha House and Erica Fatica and Rohan Shah and Jared Stergar and Ryan Pearce and Yana Sandlers",

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AU - House, Alisha

AU - Fatica, Erica

AU - Shah, Rohan

AU - Stergar, Jared

AU - Pearce, Ryan

AU - Sandlers, Yana

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Recent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here we report a step by step protocol for iPSCM metabolic characterization. The described assays cover analysis of small metabolites involved in a vital metabolic pathways.

AB - Recent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here we report a step by step protocol for iPSCM metabolic characterization. The described assays cover analysis of small metabolites involved in a vital metabolic pathways.

KW - GCMS

KW - Induced pluripotent stem cell-derived cardiomyocytes

KW - LC–MS/MS

KW - Metabolic characterization

KW - iPS-CM functional characterization

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JO - MethodsX

JF - MethodsX

M1 - 100572

ER -

A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)

Abstract

UN SDGs

Keywords

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