TY - JOUR
T1 - BSA-boronic acid conjugate as lectin mimetics
AU - Narla, Satya Nandana
AU - Pinnamaneni, Poornima
AU - Nie, Huan
AU - Li, Yu
AU - Sun, Xue-Long
PY - 2014/1/10
Y1 - 2014/1/10
N2 - We report bovine serum albumin (BSA)-boronic acid (BA) conjugates as lectin mimetics and their glyco-capturing capacity. The BSA-BA conjugates were synthesized by amidation of carboxylic acid groups in BSA with aminophenyl boronic acid in the presence of EDC, and were characterized by Alizarin Red S (ARS) assay and SDS-PAGE gel. The BSA-BA conjugates were immobilized onto maleimide-functionalized silica beads and their sugar capturing capacity and specificity were confirmed by ARS displacement assay. Further, surface plasmon resonance (SPR) analysis of the glyco-capturing activity of the BSA-BA conjugates was conducted by immobilizing BSA-BA onto SPR gold chip. Overall, we demonstrated a BSA-BA-based lectin mimetics for glyco-capturing applications. These lectin mimetics are expected to provide an important tool for glycomics and biosensor research and applications. © 2013 Elsevier Inc. All rights reserved.
AB - We report bovine serum albumin (BSA)-boronic acid (BA) conjugates as lectin mimetics and their glyco-capturing capacity. The BSA-BA conjugates were synthesized by amidation of carboxylic acid groups in BSA with aminophenyl boronic acid in the presence of EDC, and were characterized by Alizarin Red S (ARS) assay and SDS-PAGE gel. The BSA-BA conjugates were immobilized onto maleimide-functionalized silica beads and their sugar capturing capacity and specificity were confirmed by ARS displacement assay. Further, surface plasmon resonance (SPR) analysis of the glyco-capturing activity of the BSA-BA conjugates was conducted by immobilizing BSA-BA onto SPR gold chip. Overall, we demonstrated a BSA-BA-based lectin mimetics for glyco-capturing applications. These lectin mimetics are expected to provide an important tool for glycomics and biosensor research and applications. © 2013 Elsevier Inc. All rights reserved.
KW - Alizarin Red S
KW - Boronic acid
KW - Bovine serum albumin
KW - Carbohydrate
KW - Lectin
KW - Surface plasmon resonance
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U2 - 10.1016/j.bbrc.2013.12.006
DO - 10.1016/j.bbrc.2013.12.006
M3 - Article
C2 - 24326067
SN - 0006-291X
VL - 443
SP - 562
EP - 567
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -