TY - JOUR
T1 - Chemoselective immobilization of biomolecules through aqueous Diels-Alder and PEG chemistry
AU - Sun, Xue
AU - Yang, LiuChun
AU - Chaikof, Elliot L.
PY - 2008/4/14
Y1 - 2008/4/14
N2 - Aqueous Diels-Alder chemistry combined with a poly(ethylene glycol) (PEG) spacer was used to immobilize a diverse group of biomolecules onto a solid surface. Briefly, α, ω linear PEG conjugates were synthesized containing cyclopentadiene in the α position and either biotin, lactose, or protein A in the ω position. Linkers were coupled to N-maleimide (EMC)-functionalized glass substrates, and surface immobilization of biomolecules was confirmed by confocal fluorescence imaging. © 2008 Elsevier Ltd. All rights reserved.
AB - Aqueous Diels-Alder chemistry combined with a poly(ethylene glycol) (PEG) spacer was used to immobilize a diverse group of biomolecules onto a solid surface. Briefly, α, ω linear PEG conjugates were synthesized containing cyclopentadiene in the α position and either biotin, lactose, or protein A in the ω position. Linkers were coupled to N-maleimide (EMC)-functionalized glass substrates, and surface immobilization of biomolecules was confirmed by confocal fluorescence imaging. © 2008 Elsevier Ltd. All rights reserved.
KW - Biomolecules
KW - Chemical selective immobilization
KW - Diels-Alder reaction
KW - Poly(ethylene glycol)
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U2 - 10.1016/j.tetlet.2008.02.111
DO - 10.1016/j.tetlet.2008.02.111
M3 - Article
SN - 0040-4039
VL - 49
SP - 2510
EP - 2513
JO - Tetrahedron Letters
JF - Tetrahedron Letters
IS - 16
ER -