Cotranslational heme binding to nascent globin chains

A A A Komar; Aigar Kommer; Igor A. Krasheninnikov; Alexander S. Spirin

doi:10.1016/0014-5793(93)81803-8

Cotranslational heme binding to nascent globin chains

A A A Komar
, Aigar Kommer
, Igor A. Krasheninnikov
, Alexander S. Spirin

Biological, Geological, and Environmental Sciences

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

Globin synthesis in cell-free extracts of rabbit reticulocytes was carried out in the presence of 3H-labeled hemin. Sucrose gradient centrifugation analysis revealed [3H]hemin in the polyribosome fraction. The addition of puromycin resulted in the release of both [3H]hemin- and [14C]leucine-labeled polypeptide from the polyribosomes. The data suggest cotranslational folding of the globin molecule on the ribosome and cotranslational heme binding to the nascent globin chain. © 1993.

Original language	English
Pages (from-to)	261-263
Number of pages	3
Journal	FEBS Letters
Volume	326
Issue number	1-3
DOIs	https://doi.org/10.1016/0014-5793(93)81803-8
State	Published - Jan 1 1993

Keywords

Attachment
Cotranslational folding
Heme
Hemoglobin
Nascent peptide

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10.1016/0014-5793(93)81803-8

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title = "Cotranslational heme binding to nascent globin chains",

abstract = "Globin synthesis in cell-free extracts of rabbit reticulocytes was carried out in the presence of 3H-labeled hemin. Sucrose gradient centrifugation analysis revealed [3H]hemin in the polyribosome fraction. The addition of puromycin resulted in the release of both [3H]hemin- and [14C]leucine-labeled polypeptide from the polyribosomes. The data suggest cotranslational folding of the globin molecule on the ribosome and cotranslational heme binding to the nascent globin chain. {\textcopyright} 1993.",

keywords = "Attachment, Cotranslational folding, Heme, Hemoglobin, Nascent peptide",

author = "Komar, \{A A A\} and Aigar Kommer and Krasheninnikov, \{Igor A.\} and Spirin, \{Alexander S.\}",

year = "1993",

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doi = "10.1016/0014-5793(93)81803-8",

language = "English",

volume = "326",

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TY - JOUR

T1 - Cotranslational heme binding to nascent globin chains

AU - Komar, A A A

AU - Kommer, Aigar

AU - Krasheninnikov, Igor A.

AU - Spirin, Alexander S.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Globin synthesis in cell-free extracts of rabbit reticulocytes was carried out in the presence of 3H-labeled hemin. Sucrose gradient centrifugation analysis revealed [3H]hemin in the polyribosome fraction. The addition of puromycin resulted in the release of both [3H]hemin- and [14C]leucine-labeled polypeptide from the polyribosomes. The data suggest cotranslational folding of the globin molecule on the ribosome and cotranslational heme binding to the nascent globin chain. © 1993.

AB - Globin synthesis in cell-free extracts of rabbit reticulocytes was carried out in the presence of 3H-labeled hemin. Sucrose gradient centrifugation analysis revealed [3H]hemin in the polyribosome fraction. The addition of puromycin resulted in the release of both [3H]hemin- and [14C]leucine-labeled polypeptide from the polyribosomes. The data suggest cotranslational folding of the globin molecule on the ribosome and cotranslational heme binding to the nascent globin chain. © 1993.

KW - Attachment

KW - Cotranslational folding

KW - Heme

KW - Hemoglobin

KW - Nascent peptide

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U2 - 10.1016/0014-5793(93)81803-8

DO - 10.1016/0014-5793(93)81803-8

M3 - Article

C2 - 8325375

SN - 0014-5793

VL - 326

SP - 261

EP - 263

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -

Cotranslational heme binding to nascent globin chains

Abstract

Keywords

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