TY - JOUR
T1 - High-performance liquid chromatographic determination of isofraxidin in rat plasma
AU - Li, Q.
AU - Jia, Y.
AU - Sun, L.
AU - Xu, L.
AU - Tong, L.
AU - Shen, Z. D.
AU - Liu, Y. L.
AU - Bi, K. S.
PY - 2006/3/1
Y1 - 2006/3/1
N2 - For the first time a high-performance liquid chromatographic (HPLC) method, with liquid-liquid extraction and ultraviolet (UV) absorbance detection, has been developed for quantification of isofraxidin in rat plasma. The analysis was performed on a Diamonsil C18 column (200mm x 4.6mm i.d., 5 μm particle size) with acetonitrile-0.05% phosphoric acid, 26:74 (v/v), as isocratic mobile phase. The linear range was 0.05-8.0 μg mL-1 and the lower limit of quantification was 0.05 μg mL-1. The intra and inter-day relative standard deviation (RSD) for measurement of 0.25, 2.0, and 6.0 μg mL-1 quality-control (QC) samples ranged from 5.7 to 6.4% and from 6.3 to 7.9%, respectively. Accuracy, as relative error (RE), was from ±5.8% to ±7.3%. The method was validated for specificity, accuracy, and precision and was successfully used in a pharmacokinetic study of isofraxidin in rat plasma after administration of Ciwujia extract. © 2006 Friedr. Vieweg & Sohn/GWV Fachverlage GmbH.
AB - For the first time a high-performance liquid chromatographic (HPLC) method, with liquid-liquid extraction and ultraviolet (UV) absorbance detection, has been developed for quantification of isofraxidin in rat plasma. The analysis was performed on a Diamonsil C18 column (200mm x 4.6mm i.d., 5 μm particle size) with acetonitrile-0.05% phosphoric acid, 26:74 (v/v), as isocratic mobile phase. The linear range was 0.05-8.0 μg mL-1 and the lower limit of quantification was 0.05 μg mL-1. The intra and inter-day relative standard deviation (RSD) for measurement of 0.25, 2.0, and 6.0 μg mL-1 quality-control (QC) samples ranged from 5.7 to 6.4% and from 6.3 to 7.9%, respectively. Accuracy, as relative error (RE), was from ±5.8% to ±7.3%. The method was validated for specificity, accuracy, and precision and was successfully used in a pharmacokinetic study of isofraxidin in rat plasma after administration of Ciwujia extract. © 2006 Friedr. Vieweg & Sohn/GWV Fachverlage GmbH.
KW - Ciwujia extract
KW - Column liquid chromatography
KW - Isofraxidin
KW - Pharmacokinetic study
KW - Rat plasma
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U2 - 10.1365/s10337-005-0706-5
DO - 10.1365/s10337-005-0706-5
M3 - Article
SN - 0009-5893
VL - 63
SP - 249
EP - 253
JO - Chromatographia
JF - Chromatographia
IS - 5-6
ER -