TY - JOUR
T1 - One-pot glyco-affinity precipitation purification for enhanced proteomics: The flexible alignment of solution-phase capture/release and solid-phase separation
AU - Sun, Xue Long
AU - Haller, Carolyn A.
AU - Wu, XiaoYi
AU - Conticello, Vincent P.
AU - Chaikof, Elliot L.
PY - 2005/11/1
Y1 - 2005/11/1
N2 - A one-pot affinity precipitation purification of carbohydrate-binding protein was demonstrated by designing thermally responsive glyco-polypeptide polymers, which were synthesized by selective coupling of pendant carbohydrate groups to a recombinant elastin-like triblock protein copolymer (ELP). The thermally driven inverse transition temperature of the ELP-based triblock polymer is maintained upon incorporation of carbohydrate ligands, which was confirmed by differential scanning calorimetry and 1H NMR spectroscopy experiments. As a test system, lactose derivatized ELP was used to selectively purify a galactose-specific binding lectin through simple temperature-triggered precipitation in a high level of efficiency. Potential opportunities might be provided for enhanced proteomic, cell isolation as well as pathogen detection applications. © 2005 American Chemical Society.
AB - A one-pot affinity precipitation purification of carbohydrate-binding protein was demonstrated by designing thermally responsive glyco-polypeptide polymers, which were synthesized by selective coupling of pendant carbohydrate groups to a recombinant elastin-like triblock protein copolymer (ELP). The thermally driven inverse transition temperature of the ELP-based triblock polymer is maintained upon incorporation of carbohydrate ligands, which was confirmed by differential scanning calorimetry and 1H NMR spectroscopy experiments. As a test system, lactose derivatized ELP was used to selectively purify a galactose-specific binding lectin through simple temperature-triggered precipitation in a high level of efficiency. Potential opportunities might be provided for enhanced proteomic, cell isolation as well as pathogen detection applications. © 2005 American Chemical Society.
KW - Elastin-like polypeptide
KW - Glyco-affinity precipitation
KW - Glycoconjugate
KW - Protein purification
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U2 - 10.1021/pr050245g
DO - 10.1021/pr050245g
M3 - Article
C2 - 16335985
SN - 1535-3893
VL - 4
SP - 2355
EP - 2359
JO - Journal of Proteome Research
JF - Journal of Proteome Research
IS - 6
ER -