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Oriented immobilized sialyloligo-macroligand microarray

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Silaic acid is the most common terminal glycan on cell surface glycoproteins and glycolipids, involving in many biological processes. Studying interactions between multivalent sialic acid scaffolds and proteins binding to it will give incredible information in understanding the biological process the sialic acid is involved in. Here we describe chemoenzymatic synthesis of chain-end functionalized sialyllactose-containing glycopolymers with different linkages and their oriented immobilization for glycoarray and SPR-based glyco-biosensor applications.
Original languageEnglish
Title of host publicationMethods in Molecular Biology
Place of Publicationusa
PublisherHumana Press [email protected]
Pages195-206
Number of pages12
Volume1367
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
PublisherHumana Press [email protected]
Volume1367
ISSN (Print)10643745

Keywords

  • Glycan array
  • Glycopolymer
  • Hemagglutinin
  • Lectin
  • SPR
  • Sialic acid

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