Phosphorylation of 5′-O-Phosphonylmetylthymidine and Its Incorporation into the DNA of HeLa Cells

Jasko; Sidorov; R. Kondratov; Prassolov; Myasoedov; Krayevsky

Phosphorylation of 5′-O-Phosphonylmetylthymidine and Its Incorporation into the DNA of HeLa Cells

Jasko
, Sidorov
, R. Kondratov
, Prassolov
, Myasoedov
, Krayevsky

Biological, Geological, and Environmental Sciences

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Institute of Molecular Genetics RAS

Research output: Contribution to journal › Article › peer-review

Abstract

[3H]5′-O-Phosphonylmethylthymidine with a specific activity of 71 Ci/mmol was obtained by isotope exchange. Its incubation with a HeLa cell culture resulted in the formation of [3H]-labeled 5′-O-(β-phosphoryl-α-phosphonylmethyl)thymidine, 5′-O-(β,γ-diphosphoryl-α-phosphonylmethyl)thymidine, and [3H]DNA. This proved the ability of 5′-O-phosphonylmethylthymidine to undergo phosphorylation and incorporation into the DNA of human cells.

Original language	English
Pages (from-to)	23-24
Number of pages	2
Journal	Bioorganicheskaya Khimiya
Volume	24
Issue number	1
State	Published - Jan 1 1998

Keywords

3H-labeled 5′-O-phosphonylthymidine
Cells
Phosphorylation

Cite this

@article{d21a21ee9c0b4fa3b85a21f98efedaba,

title = "Phosphorylation of 5′-O-Phosphonylmetylthymidine and Its Incorporation into the DNA of HeLa Cells",

abstract = "[3H]5′-O-Phosphonylmethylthymidine with a specific activity of 71 Ci/mmol was obtained by isotope exchange. Its incubation with a HeLa cell culture resulted in the formation of [3H]-labeled 5′-O-(β-phosphoryl-α-phosphonylmethyl)thymidine, 5′-O-(β,γ-diphosphoryl-α-phosphonylmethyl)thymidine, and [3H]DNA. This proved the ability of 5′-O-phosphonylmethylthymidine to undergo phosphorylation and incorporation into the DNA of human cells.",

keywords = "3H-labeled 5′-O-phosphonylthymidine, Cells, Phosphorylation",

author = "Jasko and Sidorov and R. Kondratov and Prassolov and Myasoedov and Krayevsky",

year = "1998",

month = jan,

day = "1",

language = "English",

volume = "24",

pages = "23--24",

journal = "Bioorganicheskaya Khimiya",

issn = "0132-3423",

publisher = "Maik Nauka-Interperiodica Publishing",

number = "1",

}

TY - JOUR

T1 - Phosphorylation of 5′-O-Phosphonylmetylthymidine and Its Incorporation into the DNA of HeLa Cells

AU - Jasko, null

AU - Sidorov, null

AU - Kondratov, R.

AU - Prassolov, null

AU - Myasoedov, null

AU - Krayevsky, null

PY - 1998/1/1

Y1 - 1998/1/1

N2 - [3H]5′-O-Phosphonylmethylthymidine with a specific activity of 71 Ci/mmol was obtained by isotope exchange. Its incubation with a HeLa cell culture resulted in the formation of [3H]-labeled 5′-O-(β-phosphoryl-α-phosphonylmethyl)thymidine, 5′-O-(β,γ-diphosphoryl-α-phosphonylmethyl)thymidine, and [3H]DNA. This proved the ability of 5′-O-phosphonylmethylthymidine to undergo phosphorylation and incorporation into the DNA of human cells.

AB - [3H]5′-O-Phosphonylmethylthymidine with a specific activity of 71 Ci/mmol was obtained by isotope exchange. Its incubation with a HeLa cell culture resulted in the formation of [3H]-labeled 5′-O-(β-phosphoryl-α-phosphonylmethyl)thymidine, 5′-O-(β,γ-diphosphoryl-α-phosphonylmethyl)thymidine, and [3H]DNA. This proved the ability of 5′-O-phosphonylmethylthymidine to undergo phosphorylation and incorporation into the DNA of human cells.

KW - 3H-labeled 5′-O-phosphonylthymidine

KW - Cells

KW - Phosphorylation

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0347246645&origin=inward

UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=0347246645&origin=inward

M3 - Article

C2 - 9551197

SN - 0132-3423

VL - 24

SP - 23

EP - 24

JO - Bioorganicheskaya Khimiya

JF - Bioorganicheskaya Khimiya

IS - 1

ER -

Phosphorylation of 5′-O-Phosphonylmetylthymidine and Its Incorporation into the DNA of HeLa Cells

Abstract

Keywords

Other files and links

Cite this