Serum can act as a shear protecting agent in agitated hybridoma cell cultures

G  M Lee; J  M Savinell; B  O Palsson; Joanne Belovich

Serum can act as a shear protecting agent in agitated hybridoma cell cultures

G M Lee
, J M Savinell
, B O Palsson
, Joanne Belovich

Chemical and Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Hybridoma cells, S3H5/gamma 2bA2, were grown in spinner flasks containing RPMI 1640 media at different levels of serum. Cells in low serum media (1% fetal bovine serum) were more sensitive to shear induced by mechanical agitation than cells in high serum media (10%), indicating that serum can protect cells from shear induced by mechanical agitation. The data suggest that serum alters the physiological properties of the cells to make them more shear-resistant, rather than by changing the physico-chemical properties of medium.

Original language	English
Number of pages	593
Journal	Hybridoma
Volume	8
Issue number	6
State	Published - 1989

Cite this

@article{28fbe0962b5e46d4ab0cdac5d065ae0a,

title = "Serum can act as a shear protecting agent in agitated hybridoma cell cultures",

abstract = "Hybridoma cells, S3H5/gamma 2bA2, were grown in spinner flasks containing RPMI 1640 media at different levels of serum. Cells in low serum media (1\% fetal bovine serum) were more sensitive to shear induced by mechanical agitation than cells in high serum media (10\%), indicating that serum can protect cells from shear induced by mechanical agitation. The data suggest that serum alters the physiological properties of the cells to make them more shear-resistant, rather than by changing the physico-chemical properties of medium.",

author = "Lee, \{G M\} and Savinell, \{J M\} and Palsson, \{B O\} and Joanne Belovich",

year = "1989",

language = "English",

volume = "8",

journal = "Hybridoma",

number = "6",

}

TY - JOUR

T1 - Serum can act as a shear protecting agent in agitated hybridoma cell cultures

AU - Lee, G M

AU - Savinell, J M

AU - Palsson, B O

AU - Belovich, Joanne

PY - 1989

Y1 - 1989

N2 - Hybridoma cells, S3H5/gamma 2bA2, were grown in spinner flasks containing RPMI 1640 media at different levels of serum. Cells in low serum media (1% fetal bovine serum) were more sensitive to shear induced by mechanical agitation than cells in high serum media (10%), indicating that serum can protect cells from shear induced by mechanical agitation. The data suggest that serum alters the physiological properties of the cells to make them more shear-resistant, rather than by changing the physico-chemical properties of medium.

AB - Hybridoma cells, S3H5/gamma 2bA2, were grown in spinner flasks containing RPMI 1640 media at different levels of serum. Cells in low serum media (1% fetal bovine serum) were more sensitive to shear induced by mechanical agitation than cells in high serum media (10%), indicating that serum can protect cells from shear induced by mechanical agitation. The data suggest that serum alters the physiological properties of the cells to make them more shear-resistant, rather than by changing the physico-chemical properties of medium.

M3 - Article

VL - 8

JO - Hybridoma

JF - Hybridoma

IS - 6

ER -