TY - JOUR
T1 - Solvent properties of water in aqueous solutions of elastin-like polypeptide
AU - Ferreira, Luisa A.
AU - Cole, James T.
AU - Reichardt, Christian
AU - Holland, Nolan B.
AU - Uversky, Vladimir N.
AU - Zaslavsky, Boris Y.
PY - 2015/6/12
Y1 - 2015/6/12
N2 - The phase-transition temperatures of an elastin-like polypeptide (ELP) with the (GVGVP)40 sequence and solvent dipolarity/polarizability, hydrogen-bond donor acidity, and hydrogen-bond acceptor basicity in its aqueous solutions were quantified in the absence and presence of different salts (Na2SO4, NaCl, NaClO4, and NaSCN) and various osmolytes (sucrose, sorbitol, trehalose, and trimethylamine N-oxide (TMAO)). All osmolytes decreased the ELP phase-transition temperature, whereas NaCl and Na2SO4 decreased, and NaSCN and NaClO4 increased it. The determined phase-transition temperatures may be described as a linear combination of the solvent’s dipolarity/polarizability and hydrogen-bond donor acidity. The linear relationship established for the phase-transition temperature in the presence of salts differs quantitatively from that in the presence of osmolytes, in agreement with different (direct and indirect) mechanisms of the influence of salts and osmolytes on the ELP phase-transition temperature.
AB - The phase-transition temperatures of an elastin-like polypeptide (ELP) with the (GVGVP)40 sequence and solvent dipolarity/polarizability, hydrogen-bond donor acidity, and hydrogen-bond acceptor basicity in its aqueous solutions were quantified in the absence and presence of different salts (Na2SO4, NaCl, NaClO4, and NaSCN) and various osmolytes (sucrose, sorbitol, trehalose, and trimethylamine N-oxide (TMAO)). All osmolytes decreased the ELP phase-transition temperature, whereas NaCl and Na2SO4 decreased, and NaSCN and NaClO4 increased it. The determined phase-transition temperatures may be described as a linear combination of the solvent’s dipolarity/polarizability and hydrogen-bond donor acidity. The linear relationship established for the phase-transition temperature in the presence of salts differs quantitatively from that in the presence of osmolytes, in agreement with different (direct and indirect) mechanisms of the influence of salts and osmolytes on the ELP phase-transition temperature.
KW - And hydrogen-bond acceptor basicity
KW - Elastin-like polypeptide
KW - Hydrogen-bond donor acidity
KW - Osmolyte
KW - Phase-transition temperature
KW - Solvent dipolarity/polarizability
KW - Solvent properties
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U2 - 10.3390/ijms160613528
DO - 10.3390/ijms160613528
M3 - Article
C2 - 26075870
SN - 1661-6596
VL - 16
SP - 13528
EP - 13547
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 6
ER -