TY - JOUR
T1 - Synthetic melanin films as potential interfaces for peroxynitrite detection and quantification
AU - Kalil, Haitham
AU - Maher, Shaimaa
AU - Bose, T.
AU - Al-Mahmoud, O.
AU - Kay, C.
AU - Bayachou, Mekki
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Peroxynitrite (PON) is a highly reactive oxygen-nitrogen species that facilitates both oxidation and nitration reactions. Early reports have revealed the deleterious effects of PON on DNA, proteins, and lipids. Recent studies have suggested that melanin can act as an antioxidative therapy to scavenge the reactive oxygen-nitrogen species (RO-NS) including PON. Melanin is a natural pigment that has many physiological functions involving the neutralization of highly oxidative species. In this project, the interaction between PON and synthetic melanin has been studied. In addition, the electrochemical characteristics of the polymerized 5,6-dihydroxy indole (DHI) as a model of synthetic melanin were examined using cyclic voltammetry and electrochemical quartz crystal microbalance (EQCM). The ultraviolet-visible (UV-Vis) spectroscopy showed a significant difference in the absorbance of PON alone and in the presence of melanin films. Finally, we report on the possibility of using the DHI-melanin film as a platform for the quantitative detection of PON in solutions.
AB - Peroxynitrite (PON) is a highly reactive oxygen-nitrogen species that facilitates both oxidation and nitration reactions. Early reports have revealed the deleterious effects of PON on DNA, proteins, and lipids. Recent studies have suggested that melanin can act as an antioxidative therapy to scavenge the reactive oxygen-nitrogen species (RO-NS) including PON. Melanin is a natural pigment that has many physiological functions involving the neutralization of highly oxidative species. In this project, the interaction between PON and synthetic melanin has been studied. In addition, the electrochemical characteristics of the polymerized 5,6-dihydroxy indole (DHI) as a model of synthetic melanin were examined using cyclic voltammetry and electrochemical quartz crystal microbalance (EQCM). The ultraviolet-visible (UV-Vis) spectroscopy showed a significant difference in the absorbance of PON alone and in the presence of melanin films. Finally, we report on the possibility of using the DHI-melanin film as a platform for the quantitative detection of PON in solutions.
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U2 - 10.1149/08010.1447ecst
DO - 10.1149/08010.1447ecst
M3 - Conference article
SN - 1938-6737
VL - 80
SP - 1447
EP - 1458
JO - ECS Transactions
JF - ECS Transactions
IS - 10
T2 - 232nd ECS Meeting
Y2 - 1 October 2017 through 5 October 2017
ER -